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Schleicher and Schuell Blotting Membranes

 

Membrane

Material

Pore Size

 

Binding Capacity

Handling Properties

Information

Protran®

BA 83/85

The optimum transfer media for nucleic acids and proteins. Suitable for Southern and Western Blots, colony and plaque lifts.

Nitrocellulose

100% Pure

0.45µm (BA 85)

0.2µm

(BA 83)

0.1µm

(BA 79)

75-110µg/cm2

High binding capacity with low background providing superior signal-to-noise ratio.

There is no requirement for methanol pre-wetting.

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Optitran ®

BA-S 83/85

Designed for procedures requiring repeated stripping and reprobing of membranes. Ideal for all standard nitrocellulose protocols

Reinforced nitrocellulose

100% nitrocellulose with polyester support.

Triton-free

0.45µm

(BA-S 85)

0.2µm

(BA-S 83)

 

75-90µg/cm2

Provides a combination of exceptional handling characteristics due to the support material with excellent signal-to-noise ratio.

Recommended for repeated reprobing procedures with Southern, Western, Northerns, immunoblots, colony and plaque lifts.

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Nytran® N

Designed for Southern and Northern Blotting, colony and plaque lifts.

Moderately Charged Nylon Membrane

0.45µm

0.2µm

>400 µg/cm2

Flat, non-curling membrane with consistent membrane morphology. Ideal for applications requiring a lower positive charge than Nytran-plus

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Nytran® SuPerCharge

Very uniform pore size and distribution compared to typical nylon membranes leading to greater reproducibility of results.

Highly positively charged Nylon Membrane

0.45µm

>400 µg/cm2

Manufactured to provide high positive charge yet low background. Membrane lies flat without curling making it ideal for macroarray applications.

Suitable for radioactive or non-radioactive applications.

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Westran® S and

Westran® Clear

Westran® S is designed for protein sequencing applications

Westran Clear is recommended for  Western blotting and dot blots.

 

PVDF Membranes

 

0.2µm

Westran® S

 

0.45µm

Westran® Clear

>200 µg/cm2

Westran® S

 

125 µg/cm2

Westran® Clear

 

Extremely low backgrounds with very high protein binding capacities.

High strength of Westran Clear enables it to be used for multiple stripping and reprobing.

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