Sequencing Reagents

SequaGel^® 4, 4.25, 4.75, 6 and 8 Download Protocol

• Ready-To-Use 19:1 Denaturing DNA Gel Solutions
• Aldehyde and Ammonia Free
• Consistently Crystal Clear Gels
• Twelve Month Shelf Life at Room Temperature

SequaGels 4, 4.25, 4.75, 6 and 8 each consists of SequaGel Monomer Solution and SequaGel Complete Buffer Solution. The respective SequaGel Monomer Solution contains urea, as well as acrylamide and bis-acrylamide in the standard 19:1 ratio. SequaGel Complete Buffer Solution contains TBE and TEMED. Upon combining these two solutions, the researcher adds ammonium persulfate to form a crystal clear electrophoresis matrix with corresponding percent acrylamide (4, 4.25, 4.75, 6, or 8) containing 1X TBE (89 mM Tris Base, 89 mM Boric Acid, 2 mM EDTA, pH 8.3) and 6M Urea. Easy to use and reliable, SequaGel 4, 4.25, 4.75, 6 and 8 are well-regarded favourites of molecular biologists the world over.

Storage: SequaGel solutions are stable for one year when stored tightly capped in a dark area at room temperature (20^oC).

SequaGel^® Sequencing System Download Protocol

• Casts 19:1 Denaturing Gels up to 20% Monomer
• Aldehyde Free and Ammonia Free
• Stabilized for Long Shelf Life
• Consistently Crystal Clear Gels

The SequaGel Sequencing System consists of SequaGel Concentrate, SequaGel Diluent, and SequaGel Buffer. This system provides a convenient, dependable means for researchers to prepare gels of varying percentage. With the SequaGel Sequencing System the researcher readily prepares any commonly used gel formulation up to 20% monomer (19:1 Acrylamide/Bisacrylamide). Liter bottles of SequaGel Concentrate contain 237.5 grams of acrylamide, 12.5 grams of methylene bisacrylamide, and urea in a deionized aqueous solution. SequaGel Diluent is supplied in 450 ml and 1 liter bottles containing urea in deionized water. SequaGel Buffer is supplied in 100 ml and 200 ml bottles containing 0.89M Tris-Borate-20mM EDTA buffer pH 8.3 (10X TBE) and urea.

Storage: SequaGel Concentrate, SequaGel Diluent, and SequaGel Buffer are stable for one year when stored tightly capped in a dark area at room temperature (20^oC).

SequaGel^® XR Download Protocol

• Extended Range Gel Solution for DNA Electrophoresis
• Higher Resolution
• Rapid Running
• Available as a Premixed 2 Bottle Kit or Concentrate

National Diagnostics' SequaGel XR is specially formulated to produce greater resolution and more information -- up to 500 DNA bands on a single-load gel. SequaGel XR's optimized, proprietary formulation prevents aberrant band inversions and yields sharp, clear, highly resolved bands.

SequaGel XR runs substantially faster than traditional gels, while actually improving resolution. These results are achieved in a comfortable time-tested format, providing full interlaboratory reproducibility. By preventing band inversions, SequaGel XR bands are sharp and true. SequaGel XR provides more information by allowing greater separation between bands near the top of the gel. This effect, similar to a wedge gel, is achieved with standard spacers.

The quality and consistency of SequaGel XR is evidenced by the uniform background obtained upon drying. Band edges are sharp. Even without fixing, the gel will dry without cracking or crazing. The result is a finely-rendered, highly detailed DNA gel.

SequaGel XR is shipped as a two component system consisting of the SequaGel XR Monomer Solution and SequaGel Complete Buffer. SequaGel Complete Buffer Solution contains TBE and TEMED. No dilutions are necessary in this easy to use system. The researcher simply combines the two solutions, adds ammonium persulfate, and casts the gel. SequaGel XR is also available in the form of a 50% stock solution, SequaGel XR Concentrate.

Storage: SequaGel XR and SequaGel XR Concentrate are stable for one year when stored tightly capped, in a dark area at room temperature (20^oC).

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