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New ProteoSpin Protein Clean Up Kits from Norgen Biotek

The ProteoSpin™ product line is highly suited for protein sample preparation for a wide array of downstream applications including:

  • Mass Spectrometry
  • SDS-PAGE
  • Isoelectric Focusing
  • X-ray Crystallography
  • NMR Spectroscopy
  • Proteolytic Digestion
  • Protein Refolding Experiments

ProteoSpin™ spin columns use a proprietary-processed silicon carbide (SiC) as the chromatographic matrix. This novel man-made matrix offers unique benefits for protein sample clean-up.

  • SiC is second only to diamonds in hardness, making it capable of withstanding high-speed centrifugation for spin column applications
  • SiC is chemically inert and can tolerate extreme pH values and high temperatures
  • SiC has an innate electrostatic charge, and when processed appropriately, binds both acidic and basic macromolecules
  • SiC binds salts poorly, making it an excellent matrix for desalting and buffer exchange

Choose from current ProteoSpin™ kits. Each kit offers unique benefits for protein sample preparation.

Each kit contains ready-to-use components to process both acidic and basic protein samples. Protein capacity per spin column is 2-50 µg for Micro Kits and 0.25-8 mg for Maxi Kits.

ProteoSpin Kit Method Summary

5 Step Method

6 Step Method

9 Step Method

ProteoSpin Kit Formats

ProteoSpin™ CBED (Concentration, Buffer Exchange and Desalting) Kit — Simultaneously concentrates, provides buffer exchange and removes salts using a rapid procedure.

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New ProteoSpin™ CBED (Concentration, Buffer Exchange and Desalting) Maxi Kit — Effectively desalts and concentrates up to 8 mg of protein with an efficient, easy-to-use protocol.

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ProteoSpin™ Detergent Clean-up Kit — Provides a fast and effective procedure to remove detergents including SDS, Triton X-100, CHAPS, NP-40 and Tween 20.

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New  ProteoSpin™ Detergent Clean-up Maxi Kit — Effectively removes detergents from up to 8 mg of protein with and efficient, easy-to-use protocol.

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ProteoSpin™ Inclusion Body Isolation Kit — Three kits in one. This kit facilitates screening by providing the rapid isolation, solubilization and purification of inclusion body proteins from up to 1.5 mL bacterial culture.

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ProteoSpin Kit Ordering Information

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Testimonials

York University

 

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