ProteoSpin™
CBED (Concentration, Buffer Exchange and Desalting) Kits
-
Fast and easy processing
- Efficiently process up to 12 samples in only 20 minutes using an
easy-to-use protocol.
-
No mixing or formulation
- Solutions and protocols are provided for both acidic and basic
proteins with no need for formulation.
-
No molecular weight cutoff
- Based on an ion exchange mechanism, a broad size range of
proteins, from peptides to antibodies can be processed.
-
Proteins bind while salts are
discarded in flow-through
- SiC has an inherent low affinity for salt, but high affinity for
proteins, providing salt removal and buffer exchange
simultaneously.
Suitable for
downstream applications
- Final elution performed with volatile or non-volatile buffers
suitable for:
• Mass Spectrometry
• SDS-PAGE
• Isoelectric Focusing
• X-ray crystallography
• NMR spectroscopy
ProteoSpin™
CBED (Concentration, Buffer Exchange and Desalting) Micro Kit
Contents
Each ProteoSpin™
CBED Micro Kit provides essential components for use with the
provided protocols.
-
Column activation and wash
buffer for acidic proteins
-
pH binding buffer for acidic
proteins
-
Column activation and wash
buffer for basic proteins
-
pH binding buffer for basic
proteins
-
Elution buffer
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Neutralizer
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ProteoSpin™ SiC micro spin
columns
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Collection tubes (2 mL capacity)
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Final collection tubes (1.7 mL
capacity)
Customer-supplied Reagents and Equipment
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Benchtop microcentrifuge
-
Micropipettors
-
pH indicator paper
-
Other elution buffers
(optional)
Technical Note
Abstract
The ability of the
ProteoSpin™ CBED (Concentration, Buffer Exchange and Desalting) Kit
to concentrate and desalt dilute protein samples prepared in various
buffers was investigated.
Processing of a
dilute sample of protein with the ProteoSpin™ CBED (Concentration,
Buffer Exchange and Desalting) Kit easily yielded a 10-fold
concentration of protein with a final volume of 100 µL, with the
procedure taking less than 20 minutes to complete. The percent
recovery of protein from the columns was approximately 96%. Fitting
the data to a hyperbolic function (Figure 1) demonstrated a distinct
linear range at lower protein levels (up to 40 µg) that appears to
saturate at higher levels.
The ProteoSpin™
CBED (Concentration, Buffer Exchange and Desalting) Kit is also able
to effectively desalt protein solutions while maintaining a high
level of recovery. The following table illustrates the effectiveness
of the kit to yield a high protein recovery while simultaneously
desalting the protein sample.
Table 1:
Effectiveness of the ProteoSpin™ CBED (Concentration, Buffer
Exchange and Desalting) Kit to lower salts and concentrate protein.
Figure 1:
Relationship between Input Amount versus 1 Eluted Amount for BSA
Downloadable
CBED Application Notes
More info
Rapid Concentration
More info
Rapid Desalting
Downloadable
Instruction Manual (4MB)
Ordering
Information
