Protein Separation Reagents

ProtoPrep II Image

ProtoPrepTM II

  • Meltable Synthetic Electrophoresis Matrix
  • Melted Gel Stays Liquid at Room Temperature
  • Separate, Purify, and Recover Proteins Rapidly
  • Use Liquefied Gel Immediately for Further Processing
  • High Yields from Trypsinization

 

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The ProtoPrep II System provides a meltable synthetic gel matrix as an acrylamide replacement for the separation, purification, and recovery of proteins. After the gel is run, proteins from 10 to 220kD can be located, excised, and then recovered from the matrix by melting the gel section. The ProtoPrep II gel will melt only once and then remains liquefied. The protein of interest may be processed directly in the aqueous phase or further purified using the methods provided.

ProtoPrep II can be run in any standard vertical electrophoresis apparatus. Simply combine the ProtoPrep II concentrate, ProtoPrep II Buffer, and water, initiate and cast the gel. Gels are ready to use in 1 hour.

For maximum protein recovery, Insite, National Diagnostics¹ run-time protein visualization system is recommended for use with ProtoPrep II. Insite incorporates a unique dye/buffer combination which stains proteins during the gel run. Bands containing as little as 10 ng of protein can be detected with just a 30 minute post-run destaining in water.

ProtoPrep II is supplied in 450 ml kits containing 200ml of ProtoPrep II Concentrate, 200ml of ProtoPrep II Gel Buffer and 50 ml of ProtoPrep II Dissolution Reagent.

The buffers accompanying the ProtoPrep II kit are designed for protein electrophoresis. To carry out electrophoresis of DNA or RNA, please separately order ProtoPrep II Concentrate, ProtoPrep II Dissolution Reagent, and TTE Glycerol Tolerant Buffer.

Storage: Recommended storage of ProtoPrep II Concentrate is at 4-8oC for up to one year.

 

 

AcrylaGel and Bis-AcrylaGel Image

AcrylaGelTM
Bis-AcrylaGelTM

  • Acrylamide and Bisacrylamide Stabilized Solutions
  • Deionized and Pre-filtered
  • Aldehyde Free and Acrylic Acid Free
  • Stabilized for a Two Year Shelf Life

 

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  AcrylaGel is a ready-to-use, 30% acrylamide solution in distilled/deionized water. Because acrylamide in its crystalline form is subject to self-polymerization, the purity and consistency of polymerized AcrylaGel is superior to that obtainable with powdered acrylamide.

AcrylaGel can be crosslinked with Bis-AcrylaGel, a ready-to-use, 2% solution of methylene bisacrylamide with the same advantages as the AcrylaGel acrylamide solution. However, any powdered crosslinking reagent can be used with AcrylaGel.

AcrylaGel contains 300 grams of acrylamide per liter of solution. Bis-AcrylaGel contains 20 grams of methylene bisacrylamide per liter of solution.

Storage: AcrylaGel and Bis-AcylaGel are stable for 24 months when stored tightly capped in a dark area at room temperature (20oC).

 

Protogel Solution

37.5:1 Acrylamide to Bisacrylamide Stabilized Solution

  • Optimized for SDS-Page of Proteins (laemmli gels)
  • Consistently Crystal Clear Gels, Zeo Fluorescence
  • Stabilized for Long Shelf Life

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  National Diagnostics ProtoGel forms an electrophoresis matrix that is ideal for the separation of proteins and polypeptides.

ProtoGel is a stabilized, ready-to-use 30% (w/v) acrylamide/methylene bisacrylamide solution (37.5:1 ratio), manufactured from the highest quality materials, from which virtually all impurities have been removed.

ProtoGel has zero acrylic acid content, eliminating the fixed charges that cause band streaking. Additionally, oxidation products such as aldehydes have been removed by a selective adsorption process. Aldehydes can attack proteins, altering the structure of the sample, and thus altering Rf values. With ProtoGel, you can trust that your results will be consistent one electrophoretic run to the next.

Storage: ProtoGel is stable for 24 months when stored tightly capped in a dark area at room temperature (20oC).

 

 

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