EZ-PCR Mycoplasma Test Kit
The newly developed EZ-PCR Mycoplasma Test Kit is a highly sensitive
PCR assay which detects all Mycoplasma species that may
contaminate tissue culture samples as well as achoplasma and
spiroplasma species. It is a quick, simple and reliable and
cost-effective method for regularly monitoring cells for mycoplasma
detection.
Ordering
Information
| Order Code |
Unit |
Description |
Price |
|
K1-0210 |
Each |
EZ-PCR Mycoplasma Test Kit, 20 assays |
£130.00 |
|
K1-0212 |
Kit |
EZ-PCR Mycoplasma Test Kit, (10 Assays) |
£72.50 |
Assay Characteristics
EZ-PCR Mycoplasma Test Kit is composed of a single tube PCR mix. It
does not require special equipment and can be performed in any lab
with a thermal cycler and electrophoresis equipment.
Running the PCR reaction products on an agarose gel will result in a
270bp band.
All of the ingredients needed for the PCR step are in a pre-mixed
reaction solution so there is no need to worry about buffer, enzyme
and dNTP concentrations. The primers used in the kit are derived
from a highly conserved region within the 16S rRNA gene and can
detect very low levels of contamination.
Working Procedure
Samples are centrifuged in a microcentrifuge tube. The pellets
obtained are suspended in a unique DNA-release buffer and the DNA is
amplified by PCR.
The time taken for preparing the sample for PCR is less than 10
minutes.
Kit Contents
The kit contains Buffer Solution, PCR mix and a Positive Control
sufficient for 20 tests.
A
downloadable PDF file with detailed instructions is included with
each kit.
Detailed Info –
Myco-pdf
Related Products
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shown to be effective in the elimination of mycoplasma species that
account for 90% of contamination found in cell cultures.
Antibiotics for the Treatment of Cultures Contaminated with
Mycoplasma
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for Disinfecting Water Baths in CO2 Incubators
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