National Diagnostics Buffers

 ProtoGel™ Buffers

The use of ProtoGel Resolving Buffer and ProtoGel Stacking Buffer will ensure the purity and performance of your lamelli gels. ProtoGel Resolving Buffer forms gels of 0.375M Tris-HCl and 0.1% SDS pH 8.8. ProtoGel Stacking Buffer forms gels of 0.125M Tris-HCl and 0.1% SDS, pH 6.8.

Protein Loading Buffer Blue (2X)

This is a ready-to-use buffer for the preparation of protein samples to be separated in a SDS-PAGE gel. Loading buffer blue (2X) contains bromophenol blue as a tracking dye to monitor the sample during the run. Loading buffer blue (2X) contains 0.5M Tris-HCl (pH 6.8), 4.4% (w/v) SDS, 20% (v/v) Glycerol, 2% (v/v) 2- mercaptoethanol and bromphenol blue in distilled/deionised water.

SSC Buffer (20X)

SSC Buffer (20X) is a concentrated solution of sodium chloride-sodium citrate in distilled/deionised water. SSC Buffer is widely used in nucleic acid blotting and hybridization protocols.

SSC Concentrate (20X) is already in solution, which eliminates the weighing, mixing, and adjusting of pH necessary with powdered buffers. To use 20X SSC, simply dilute with an appropriate amount of distilled/deionised water. The 20X formulation allows for easy dilutions to all needed concentrations.

SSC Buffer Concentrate (20X) is supplied in 1 litre and 4 litre bottles containing 3M sodium chloride and 0.3M sodium citrate (pH 7.0).

TAE Buffer (50X)

TAE Buffer (50X) is a concentrated Tris-Acetate-EDTA solution in distilled/deionised water. TAE Buffer (50X) is especially useful in agarose gel electrophoresis when the DNA of interest is to be isolated after electrophoresis.

TAE Buffer (50X) is already in solution, which eliminates the weighing, mixing, and adjusting of the pH necessary with powdered buffers. To use 50X TAE, simply dilute with an appropriate amount of distilled/deionised water.

TBE Buffer (10X or 5X)

National Diagnostics' TBE Buffer is a concentrated buffer solution of Tris-Borate-EDTA in distilled/deionised water. This solution is widely used in the preparation of nucleic acid electrophoresis gels.

TBE Buffer is already in solution, which eliminates the weighing, mixing, and adjusting of the pH necessary with powdered buffers. TBE is ready-to-use as a 10X or 5X concentrate, or it can be diluted with an appropriate amount of distilled/deionised water.

TE Buffer (100X)

TE Buffer is a 100X concentrated solution of 1 M Tris-HCl, pH 8, with 100 mM EDTA. This buffer is the standard for DNA and RNA purification, processing and storage. National Diagnostics' TE buffer is produced to the same exacting standards that have made all our buffers trusted by molecular biologists worldwide.

Tris-Glycine Electro-Blotting Buffer (10X)

Tris-Glycine Electro-blotting Buffer (10X) is a concentrated solution in distilled/deionised water. This buffer is intended to act as the tank buffer in Western electro-blotting procedures. It is also the recommended buffer for use with nitrocellulose and nylon membranes. Tris-Glycine Electro-blotting Buffer (10X) is already in solution which eliminates the weighing, mixing and adjusting of the pH necessary with powdered buffers. To prepare 1 litre of working strength buffer, add 100 ml of Tris-Glycine Electro-blotting Buffer to 200 ml of methanol and 700 ml of distilled/deionised water.

Tris-Glycine Electro-blotting Buffer (10X) is supplied in 1 and 4 litre bottles containing 0.20M Tris base and 1.50M glycine.

Tris-Glycine-SDS PAGE Buffer (10X)

SDS-PAGE Buffer (10X) is a concentrated solution of Tris-Glycine-SDS in distilled/deionised water. This buffer is especially intended for use as the running buffer in SDS polyacrylamide gel electrophoresis (PAGE) for proteins. Tris-Glycine-SDS PAGE Buffer (10X) is already in solution, which eliminates the weighing, mixing, and adjusting of the pH necessary with powdered buffers.

Tris-Glycine-SDS PAGE Buffer (10X) is supplied as a 10X Concentrate in 1 and 4 litre bottles. Each bottle contains 0.25M Tris base, 1.92M glycine, and 1% (w/v) SDS.

 TTE Glycerol Tolerant Buffer (20X)

National Diagnostics' TTE Glycerol Tolerant Buffer (20X) is a concentrated Tris-Taurine-EDTA buffer solution in distilled/deionised water. This buffer may be used in the preparation of the same sequencing gels that you are now running, resulting in a glycerol tolerant gel.

TTE (20X) eliminates the band distortion associated with DNA samples in glycerol. With TTE Buffer (20X), DNA/glycerol samples can be used without diluting the samples.

National Diagnostics' TTE Glycerol Tolerant Buffer (20X) is supplied in 1 litre bottles, containing 1.78M Tris base, 0.57M Taurine, and 10mM Na₂EDTA.

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