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Acrylamides
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SequaGel®
Sequencing System
- Casts 19:1 Denaturing Gels up to
20% Monomer
- Aldehyde Free and Ammonia Free
- Stabilized for Long Shelf Life
- Consistently Crystal Clear Gels
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Download Protocol
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The SequaGel Sequencing
System consists of SequaGel Concentrate,
SequaGel Diluent, and SequaGel Buffer. This
system provides a convenient, dependable
means for researchers to prepare gels of
varying percentage. With the SequaGel Sequencing
System the researcher readily prepares any
commonly used gel formulation up to 20%
monomer (19:1 Acrylamide/Bisacrylamide).
Liter bottles of SequaGel Concentrate contain
237.5 grams of acrylamide, 12.5 grams of
methylene bisacrylamide, and urea in a deionized
aqueous solution. SequaGel Diluent is supplied
in 450 ml and 1 liter bottles containing
urea in deionized water. SequaGel Buffer
is supplied in 100 ml and 200 ml bottles
containing 0.89M Tris-Borate-20mM EDTA buffer
pH 8.3 (10X TBE) and urea.
Storage: SequaGel Concentrate,
SequaGel Diluent, and SequaGel Buffer are
stable for one year when stored tightly
capped in a dark area at room temperature
(20oC).
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SequaGel®
XR
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Extended Range Gel Solution for DNA
Electrophoresis
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Higher Resolution
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Rapid Running
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Available as a Premixed 2 Bottle Kit
or Concentrate
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Download Protocol
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National Diagnostics'
SequaGel XR is specially formulated to produce
greater resolution and more information
-- up to 500 DNA bands on a single-load
gel. SequaGel XR's optimized, proprietary
formulation prevents aberrant band inversions
and yields sharp, clear, highly resolved
bands.
SequaGel XR runs substantially
faster than traditional gels, while actually
improving resolution. These results are
achieved in a comfortable time-tested format,
providing full interlaboratory reproducibility.
By preventing band inversions, SequaGel
XR bands are sharp and true. SequaGel XR
provides more information by allowing greater
separation between bands near the top of
the gel. This effect, similar to a wedge
gel, is achieved with standard spacers.
The quality and consistency
of SequaGel XR is evidenced by the uniform
background obtained upon drying. Band edges
are sharp. Even without fixing, the gel
will dry without cracking or crazing. The
result is a finely-rendered, highly detailed
DNA gel.
SequaGel XR is shipped
as a two component system consisting of
the SequaGel XR Monomer Solution and SequaGel
Complete Buffer. SequaGel Complete Buffer
Solution contains TBE and TEMED. No dilutions
are necessary in this easy to use system.
The researcher simply combines the two solutions,
adds ammonium persulfate, and casts the
gel. SequaGel XR is also available in the
form of a 50% stock solution, SequaGel XR
Concentrate.
Storage: SequaGel XR
and SequaGel XR Concentrate are stable for
one year when stored tightly capped, in
a dark area at room temperature (20oC).
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AutoMatrixTM
4.5
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Best All Around Gel Matrix for the ABI
377
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Maximum Separation and Read Length,
Low Background
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Quality Controlled for Purity and Performance
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Easy to Use: Just Add TBE and Initiator
and Cast Gel
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Download Protocol
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AutoMatrix consistently
provides read lengths greater than 700 bases
on the ABI 377 automated sequencer. Combined
with 10X TBE, AutoMatrix forms a gel with
29:1 acrylamide to bis-acrylamide in 6M
urea. Applied Biosystems has shown this
formulation to give the best separation
and read lengths over the widest range of
applications with the ABI 377 automated
sequencer. Reliable, economical, and ultra-pure,
AutoMatrix 4.5 is the optimal formulation
for automated sequencing on the ABI 377.
AutoMatrix is made
stable by the incorporation of a gaseous
inhibitor that prevents the self polymerization
to which solid acrylamide is susceptible.
It also inhibits the accumulation of oxidation
products such as acrylic acid. All of this
results in crystal clear gels of easily
reproducible molecular composition. Identical
pore sizes guarantee consistent, reliable
electrophoretic runs and Rf values.
Storage: AutoMatrix
4.5 is stable for 12 months when stored
tightly capped in a dark area at room temperature
(20oC).
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SequaGel®
MD
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For the Detection of Minor Mutational
Differences
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Point Mutation Analysis
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SSCP Analysis
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Heteroduplex Analysis
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Download Protocol
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SequaGel MD permits
minor mutational differences in DNA sequences
to be detected as a high resolution relative
mobility (Rf) shift. SequaGel MD is a proprietary
formulation, supplied as a 2X stock, designed
to resolve sequence related differences
by SSCP (Single Strand Conformational Polymorphism)
and Heteroduplex Analysis.
DNA mutations, or sequence
modifications, are readily associated with
specific disease states. Since a variation
of a single nucleotide in a sequence may
indicate a significant genetic anomaly,
an extremely sensitive method to analyze
these mutations is necessary.
Two principle methods
of conformational analysis have been developed,
SSCP Analysis and Heteroduplex Analysis.
SequaGel MD is designed to run SSCP samples
in the 100-300 nucleotide range at a 0.5X
concentration. Small fragments may be better
resolved by using higher concentration (0.75X)
gels. Larger fragments may be better resolved
by running lower concentration (0.4X) gels.
SequaGel MD is designed to run heteroduplex
analysis on DNA fragments up to 900 bases
at a 1X concentration.
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AccuGelTM
19:1
AccuGelTM
29:1
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Premixed Acrylamide:Bisacrylamide Solutions
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Stabilized for a Two Year Shelf Life
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Consistently Crystal Clear Gels
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Now available in 30% or 40% concentration
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Download Protocol
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AccuGel 19:1 and AccuGel
29:1 are ready-to-use monomer solutions
for the preparation of electrophoresis gels
for DNA or RNA. AccuGel 19:1 contains 19
grams of acrylamide per gram of methylene
bisacrylamide (MBA) cross-linker. AccuGel
29:1 contains 29 grams of acrylamide per
gram MBA.
Available in 30% or
the popular 40% concentration, these solutions
are ideal for the separation of double stranded
DNA fragments. AccuGel 19:1 is best suited
for the range of molecular size less than
100 bp, while AccuGel 29:1 is best suited
for fragments between 100 and 1000 bp. With
the addition of urea, AccuGel solutions
will also formulate superior sequencing
gels.
Storage: AccuGel 19:1
and AccuGel 29:1 are stable for 24 months
when stored tightly capped in a dark area
at room temperature (20oC).
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© 2010 Geneflow Ltd. All rights reserved.
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