EZ-RNA
ll Total RNA isolation Kit
EZ-RNA II is a
complete kit with ready-to-use reagents for the isolation of total RNA
from samples of human, animal, plant, yeast, bacterial and viral origin.
EZ-RNA II is based on disruption of cells in guanidine
thiocyanate/detergent solution, followed by organic extraction and
alcohol precipitation of the RNA, and which allows simultaneous
processing of a large number of samples.
1-Bromo-3-chloropropane (BCP) replaces chloroform, a highly volatile and
toxic reagent used in molecular biology. Substituting BCP for chloroform
in the EZ-RNA II kit reduces toxic material handling without any reverse
effects on the quality of isolated RNA, DNA or proteins.
The resulting RNA is suitable for the
isolation of Poly A+ RNA or for Northern Blotting, Dot Blotting, in
vitro Translation, Molecular Cloning, RT-PCR and RNase Protection
Assays, or other analytical procedures. DNA and proteins can also be
recovered from the interphase and the organic phase of the same sample.
For detailed method –
click here
