EZ-RNA ll Total RNA isolation Kit
EZ-RNA II is a complete kit with ready-to-use
reagents for the isolation of total RNA from samples of human,
animal, plant, yeast, bacterial and viral origin. EZ-RNA II is based
on disruption of cells in guanidine thiocyanate/detergent solution,
followed by organic extraction and alcohol precipitation of the RNA,
and which allows simultaneous processing of a large number of
samples.
1-Bromo-3-chloropropane (BCP) replaces chloroform, a
highly volatile and toxic reagent used in molecular biology.
Substituting BCP for chloroform in the EZ-RNA II kit reduces toxic
material handling without any reverse effects on the quality of
isolated RNA, DNA or proteins.
The resulting RNA is suitable for the isolation of
Poly A+ RNA or for Northern Blotting, Dot Blotting, in vitro
Translation, Molecular Cloning, RT-PCR and RNase Protection Assays,
or other analytical procedures. DNA and proteins can also be
recovered from the interphase and the organic phase of the same
sample.
For detailed method –
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