TRANSFECTION
Do you need to switch from FuGENE®
6?
Achieve Comparable Performance with TransIT-LT1 reagent
TransIT-LT1 Reagent is a high efficiency, low toxicity
reagent
TransIT-LT1 reagent gives comparable performance
to FuGENE® 6
The Proof is in the
Performance
The indicated cell lines were transfected in duplicate
with 1 µg of a luciferase expression vector per well of a
12-well plate using either 3 µl of the TransIT®-LT1 or
FuGENE® 6 Reagents. Cells were harvested 24 hours
post-transfection
Please contact Geneflow Technical Support to request your
free sample!
Ordering
Information
Transit 2020 Transfection Reagent providing
superior results on hard-to-transfect cells.
This reagent has been used successfully by researchers for
transfection of cells that are traditionally difficult, such
as primary mouse embryonic fibroblasts, mouse primary
embryonic-stem cell-derived cardiomyocytes, primary human
airway epithelial cells, T-98G (human glioblastoma cell
line), MCF-7, H522, 2b4.11 (T lymphocyte hybridoma cell
line) and human ES derived neural progenitor cells.
Researcher feedback shows that TransIT®-2020 has
outperformed competing transfection reagents such as
Fugene®HD and Lipofectamine™ 2000 to name a few.
More Transit 2020 information
Ordering Information – go to products E7-0142/144/146/148 in
Transfection Reagents
New Transfection Brochure
LabelIT NUCLEIC ACID LABELLING KITS
Efficient, direct, non-enzymatic labelling of DNA and RNA
- Covalently attach CX-rhodamine, fluorescein,
digoxin, biotin, Cy™3,
Cy™5, dinitrophenol (DNP), and TM-rhodamine labels to
nucleic acid bases within DNA or RNA
- Simple protocol requiring only 1 reagent
- Reactions are non-destructive and easy to
control
- Supplied standard Labeling protocol will yield
Labeling efficiencies of approximately one Label every
20-60 base pairs of double-stranded DNA
- Suitable for generation of FISH probes
More info – LabelIt Nucleic Acid Labelling Kit
LabelIT DELIVERY CONTROLS – PLASMID AND RNAi
Label IT® Plasmid Delivery Controls
Fluorescently labelled plasmid ideal for In Vitro and In Vivo tracking
- Enables easy visualisation of transfected cells and assesses delivery efficiency using fluorescent microscopy
- Suitable for co-delivery experiments with functional plasmids
- Supplied as a ready to use 0.5 mg/ml concentration for both In Vitro and In Vivo tracking studies
Label IT® RNAi Delivery Controls
Fluorescently labeled RNAi duplexes ideal for In Vitro or In Vivo tracking
- Easy visualisation of transfected cells and assesess delivery efficiency using fluorescent microscopy
- Inert - Not known to target any mammalian genes or cause off-target effects
- Suitable for co-delivery experiments with functional siRNA
- Supplied as a ready to use 10 µM stock solution with a 10X RNAi Dilution Buffer.
More Info – LabelIt delivery Controls
STEM CELL MEDIA PRODUCTS 
New range of media for feeder-free and
feeder-dependent culture
hES Cell based clinical applications are currently limited
by xeno-contamination during the in-vitro derivation and
propagation phases. Xeno Free (XF, or Animal Component-Free,
ACF) media are therefore an essential element in the
development of regenerative stem cell therapies.
NutriStem™ hESC XF - is a ready-to-use, complete
medium containing Human Serum Albumin (HSA), specifically
optimized for hESC feeder-independent conditions.
AF NutriStem™ hESC XF without HSA was optimized
for use on feeder cells such as Mouse Embryo Fibroblasts
(MEF) or human foreskin fibroblasts (HFF)
More NutriStem Information
Please contact Geneflow Technical Support for additional
technical information and sample requests
BLOOD/PLASMA/SERUM SAMPLE PREPARATION
KITS
Rapid Isolation of microRNA, RNA, circulating
nucleic acids and DNA
Norgen's line of blood/plasma/serum sample preparation
products provide fast, robust, reliable and simple
procedures for isolating and purifying high quality
macromolecules such as DNA, RNA, circulating nucleic acids
and microRNA from a wide range of sample volumes.
Genomic DNA can be isolated from blood inputs ranging
from as little as 10 µL to as much as 10 mL. Circulating
nucleic acids, including DNA, RNA, small RNAs and viral DNA,
can be isolated from plasma or serum inputs ranging from as
little as 0.5 mL to as much as 5 mL.
More Norgen Information
Please contact Geneflow Technical Support for additional
technical information and sample requests
THERMAL CYCLER TRIPLE BLOCK
SensoQuest Thermal Cyclers with increased block
options
The SensoQuest thermal cyclers are now available with 3 x
21 0.2ml Triple Blocks which allow 3 independent PCR runs.
The minimum volume of reagent is 10µl and all blocks are
separately monitored and controlled. This complements the 96
well and 48 well blocks which are also available.
The unique quick block changing system means that an
alternative block can be used within 10 seconds!
More SensoQuest Information
